Immunomodulatory, antileishmanial and phytotoxicity of Arisaema Jacquemontii Blume plant extracts

ArisaemajacquemontiiBlume (Araceae) is reputed for its physiological properties in the folk medicine system and used in Chinese herbal medicine as anti-convulsant. Methanolic extracts of roots and fruits, the chloroform extracts of roots and shoots of ArisaemajacquemontiiBlume were investigated for immunomodulatory,antileishmanialand phytotixic activities. Immunomodulatory activitywas investigated using luminol based chemiluminescence assay. Present study demonstrated the effect of these extracts on ROS production of human whole blood and isolated polymorphoneutrophils after activation with Serum opsonized Zymosan. Methanol and chloroform extracts of roots of the plant possess significant inhibitory activity while methanol extract of fruits and chloroform extract of shoots of the plant possess moderate inhibitory activity.Antileishmanial activities of the chloroform and methanol extracts were evaluated by 96-well micro titer plate method. The chloroform extract of the roots of the plant have shown significant antileishmanial activity while the methanol extracts of the shoots and fruits have shown low antileishmanial activity. Phytotoxic activities of same extracts were tested against the Limnaminorusing themodified protocol of McLaughlin bioassay. Methanolicextracts of fruits and roots and chloroform extract of shoots and roots were studied for their phytotoxic activity. The chloroform extract of shoots of the plant showed 100 % inhibition at concentration of 1000μg/mL while chloroform extract of the roots and methanol extract of fruits of the plant showed moderate activity at the highest dose.